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Document Type |
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Project |
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Document Title |
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Crash and the reform of the "d. N. A." In patients with leukemia and Amfum: and its relation to how patients respond to chemotherapy تحطم وإصلاح الـ"د.ن.أ." في مرضى سرطان الدم واللمفوم : وعلاقة ذلك بمدى استجابة المرضى للعلاج الكيميائي |
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Document Language |
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Arabic |
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Abstract |
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Recent evidence indicates clearly that there is a direct relationship between the extent of break, "DNA" of cancer cells caused by chemotherapy and the patient's response to treatment and disease progression. In this study, the use of IT electrophoresis of the cell one (technical comet) to identify the size of the broken DNA in the cells of 30 patients infected with different types of cancer of the blood and in different stages of the disease and to link the size of the broken DNA and the condition and extent of response to chemotherapy. These include patients 9 leukemia blood spinal sharp 0.6 leukemia blood spinal chronic 0.7 leukemia Blood sharp Borema lymph 0.2 leukemia blood lymphocytic chronic, 3 to Mfum Hodgkin, one patient each from Mfum non-Hodgkin's to Mfum Birkett and Ngayoom Multiple. And this study focused on the knowledge and compare the size of broken DNA in the cells of each patient as much as possible at diagnosis before treatment, after treatment and during recovery and relapse, and when linked to the extent of patient response or resistance to chemotherapy. Defined amount of DNA broken that the amount left nucleus and left outside to give a guilty head is the nucleus and DNA Crusher is the tail of the comet and to determine the extent of the amount of DNA broken relied on four criteria of the comet, and these standards is the length of the tail, the percentage of DNA in the header of the comet and its tail as well as the determination of the tail (the amount of DNA in the tail × tail length). The software used analysis of the comet and give the results automatically.
The results showed that there was a significant increase in broken DNA in the cells of patients who received treatment in the healing phase cells compared to patients who did not receive treatment or in the process of relapse. The increase breaks the DNA more than in the cells of patients with leukemia acute Paorma lymph T, followed by B and then cell leukemia acute followed by a Mfum Birkett (and the percentage of DNA in the tail are respectively 67.8, 65.2, 63.9, 50.2%). Has increased all the criteria tail of the comet in the cells of the treatment and entered the recovery phase compared with those not receiving treatment or in the process of relapse. And increased the determination of the comet tail and was Zaadthokpr what is in the cells of patients with acute leukemia and lymphoma Borema T cells and then acute myeloid leukemia (26.4, 25.5, 22.1, respectively).
This is a time when the broken DNA in the cells of patients at the setback or before chemotherapy is very little or negligible.
From this it follows that the breakage of DNA in the cells of patients with leukemia and Amfum and follow up on this break-up technology, electrophoresis of the cell one can be used as a standard vital to the debate over the response and the sensitivity of patients with leukemia and Amfum to chemotherapy, which leads to a significant increase in broken DNA in the case of response of patients to the contrary setback in the case of non-response. Thus, the corresponding DNA breaks in patients with cancer of the blood can be helpful in identifying patients respond to treatment or not and thus, prepare and give the proper medication. |
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Publishing Year |
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1421 AH
2001 AD |
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Added Date |
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Wednesday, April 30, 2008 |
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Researchers
| محمد حسن قاري | qari, mohammed hassan | Investigator | Doctorate | |
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